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Բաժանորդագրվեք մեր ազդագրին և ստացեք նոր ապրանքների ու հատուկ առաջարկների մասին տեղեկություններ, ինչպես նաև՝ գեղեցկության խորհուրդներ և այլ անակնկալներ:
13 Փետրվար, 2018
Essential Oil from Thymus vulgaris L. as Antimicrobial Agent
ASM MICROBE 2017 General Meeting, New Orleans, LA, USA

 Authors N. Sahakyan, M. Petrosyan, A. Avetisyan, A. Markosian, S. Aloyan, A. Babayan, A. Trchounian; Yerevan State Univ., Yerevan, Armenia 

Abstract

The aim of the investigation was to study the antimicrobial activity of essential oil from Thymus vulgaris, grown at the altitude of 1700 -1800 meters above sea level (Aragyugh, Armenia) and harvested during the blossoming period (July, 2015). Antimicrobial activity was determined by the agar diusion method. Gram-positive (Bacillus subtilis WT-A; Staphylococcus aureus MDC 5233) and Gram-negative (E. coli VKPM-M17; Pseudomonas aeruginosa GRP3 and ampicillin-resistant E. coli dhpα-pUCl8) bacteria and yeasts (Candida albicans WT-174 and Debariomyces hansenii WT) were tested. The gas chromatography-mass spectrometry setup was used to reveal the chemical composition of essential oils. The 20 substances were identied in the T. vulgaris essential oil among which carvacrol (28.4 %), p-cymene (18.5 %) and γ-terpinene (15.3 %) were the main constituents. Gram-positive bacteria were more sensitive to essential oils than Gram-negatives. The minimal inhibitory concentration (MIC) of T. vulgaris essential oil was 0.195 mg/mL against St. aureus and was 1.565 mg/mL against B. subtilis. MIC values of oil under investigation against all tested Gram-negative bacteria, including antibiotic resistant, E. coli was 6.25 mg/ml. The action of the essential oil was bactericidal. Tested fungi were also sensitive against oil components: MIC value was 1.56 mg/mL against both tested yeasts. The obtained data show that thyme essential oil can be useful natural agents for cosmetics, medicine and food as antimicrobial agent. This study was done in the frame of research support from State Committee of Science, Ministry of Education and Science of Armenia.

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